AcrB is essential for bile acid tolerance in Helicobacter pylori

Resistance to bile acids is a necessary ability for bacteria that live in the gastrointestinal tract, such as Helicobacter pylori. The role of bacterial drug efflux system important acid tolerance. Using H. pylori JP26 strain and JP26AcrB deficient AcrB relationship between tolerance was investigated. Bacterial susceptibility measured by agar dilution method. To examine acids, intracellular accumulation radiolabeled chenodeoxycholic with liquid scintillation counter. investigate effect on cell adhesion bacteria, were attached AGS cells supplemented acid, number counted. Bile resistance significantly lower mutant than wild-type strain. strains showed significant increase 14C - labeled bacteria. In terms attachment cells, Furthermore, could not be identified. Our results reveal plays an resistance. We also suggest drugs targeting may new treatment